High frequency ultrasound scattering from microspheres and single cells.
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To assess the proportion of cells in a volume undergoing structural changes, such as apoptosis, using high frequency ultrasound (20--100 MHz) requires the development of a theoretical model of scattering by any arbitrary cell ensemble. A prerequisite to building such a model is to know the scattering by a single cell in different states. In this thesis, a model for the high frequency acoustic scattering by one cell is proposed. A method for deducing the backscattering frequency response from a single sub-resolution scatterer was also devised. Using this method, experimental measurements of backscatter from homogeneous microspheres and single, viable eukaryotic cells were acquired and compared with Faran-Hickling scattering theory and the proposed cell scattering model, respectively. The resonant features observed in the backscatter frequency response of microspheres corresponded accurately to theoretical predictions. The agreement was not as good between the cell model and the measured backscatter from cells.
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“ A model based upon pseudo regular spacing of cells combined with the randomisation of the nuclei can explain the significant changes in high-frequency ultrasound signals during apoptosis,” Ultrasound Med.
Biol. 28, – /S(01)X Surface modes and acoustic scattering of microspheres and ultrasound contrast agents for modeling high frequency ultrasound scattering by spherical objects,” cells are streamed single Comparison of microsphere models for calculation of backscattered ultrasound.
High-frequency ultrasound scattering from microspheres and. single :// High frequency ultrasound backscatter signals from sea urchin oocytes were measured using a 40 MHz transducer and compared to numerical simulations.
The Faran scattering model was used to calculate the ultrasound scattered from single oocytes in suspension.
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The urchin oocytes are non-nucleated with uniform size and biomechanical properties; the backscatter from each cell is similar High frequency ultrasound scattering from mixtures of two different cells lines: tissue characterization insights M.C.
Kolios and G.J. Czarnota () In 11th International Symposium on Advanced Biomedical Ultrasound, Sendai, Japan, pp. Introduction. Focused ultrasound has been shown to induce tissue ablation through thermal or mechanical mechanisms –.Ultrasonic thermal therapy has been widely studied for the treatments including uterine fibroids and solid tumors –.In comparison, mechanical effects, mainly cavitational effects, have been avoided for tissue ablation because of the difficulty in controlling and Introduction.
Details High frequency ultrasound scattering from microspheres and single cells. FB2
Ultrasound imaging is the most frequently used clinical imaging modality, accounting for almost 25% of all imaging procedures (Forsberg ).Recent advances in transducer technology and electronics have increased ultrasonic frequencies to 20 to 60 MHz, providing better image resolution at the expense of reduced ultrasound penetration depth (Foster et al.
A study of high frequency ultrasound scattering from non-nucleated biological specimens Omar Falou, Ralph E. Baddour, George Nathanael, Gregory J. Czarnota, J.
Description High frequency ultrasound scattering from microspheres and single cells. PDF
Carl Kumaradas and Michael C. Kolios (), The Journal of the Acoustical Society of America (5): ELEL Ultrasonic backscatter coefficient (BSC) measurements were performed on K cell pellet biophantoms with cell concentrations ranging from to in the 10–42 MHz frequency bandwidth.
Three scattering models, namely, the fluid-filled sphere model (FFSM), the particle model (PM), and the structure factor model (SFM), were compared for modeling the scattering from an ensemble of High-resolution imaging of the whole mouse brain is further challenged by acoustic distortions and attenuation of high-frequency ultrasound waves by the skull.
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